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目的探讨DNA倍体及细胞增殖抗原标记物Ki67对喉鳞状细胞癌预后预测的价值。方法采用Feul-gen染色和免疫组化方法,对298例喉鳞状细胞癌石蜡标本DNA倍体情况及Ki67表达进行分析。结果喉鳞状细胞癌组织中存在着DNA非整倍体肿瘤和DNA二倍体肿瘤;DNA非整倍体肿瘤中Ki67表达高于DNA二倍体肿瘤中的表达,结果差异有统计学意义(P<0.05);进一步经COX回归多因素分析发现喉鳞状细胞癌中DNA倍体的不同和Ki67表达的高低是影响肿瘤死亡的独立因素(P=0.003,P=0.007)。结论在喉鳞状细胞癌中,DNA倍体和Ki67表达水平与颈部淋巴结转移、肿瘤复发及患者生存率相关,是判断喉鳞状细胞癌患者预后估计的提示性依据。
Objective To investigate the value of DNA ploidy and Ki67 antigen in predicting the prognosis of laryngeal squamous cell carcinoma. Methods DNA ploidy and Ki67 expression in 298 specimens of laryngeal squamous cell carcinoma were analyzed by Feul-gen staining and immunohistochemistry. Results DNA aneuploidy and DNA diploid tumors were found in laryngeal squamous cell carcinoma tissues. The expression of Ki67 in DNA aneuploid tumors was higher than that in DNA diploid tumors, and the difference was statistically significant ( P <0.05). Furthermore, multivariate analysis by COX regression showed that DNA ploidy and Ki67 expression in laryngeal squamous cell carcinoma were independent factors affecting tumor death (P = 0.003, P = 0.007). Conclusion The expression of DNA ploidy and Ki67 in laryngeal squamous cell carcinoma is related to cervical lymph node metastasis, tumor recurrence and patient survival rate, which is a suggestive basis for judging the prognosis of laryngeal squamous cell carcinoma.