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多次采用根瘤切片法直接从野生的马桑根瘤中分离内生菌纯培养物均未获成功；但用同样方法从温室里人工接种形成的马桑根瘤中却分离到大量纯培养物。其中２７３株经盆栽回接后发现有２４株能侵染结瘤；少数菌株还通过半固体斜面和珍珠岩盆栽回接成功。对１０株纯培养物表型特性的研究表明，它们均具有Ｆｒａｎｋｉａ的形态和培养特征；生理类型除有Ａ、Ｂ两型外，尚出现有一类Ａ、Ｂ混合型，其中凡属Ｂ型菌株均能侵染结瘤，Ａ型则不能，两株Ａ、Ｂ混合型中有１株能结瘤，另１株却不能；碳、氮源利用特性和其它Ｆｒａｎｋｉａ基本相似；细胞壁组分既有Ⅲ型也有Ⅱ型；全细胞鉴别性糖大多为木糖和半乳糖，少数菌株只含有半乳糖或木糖，或含有半乳糖、木糖和阿拉伯糖。ＤＮＡ中Ｇ＋Ｃｍｏｌ％为６５％～７４％。
Numerous pure cultures of Endomyces were isolated from nodules of wild Maussane nodules by multiple nodulation methods. However, a large number of pure cultures were isolated from the mulberry root nodules formed by manual inoculation in the greenhouse using the same method. Among them, 27 of the 273 plants were found to be backcrossing by potting and 24 were found to be able to infect nodulation. A few strains were also successfully connected by semi-solid bevel and perlite potted plants. The study on the phenotypic characteristics of 10 pure cultures showed that both of them had the morphological and cultural features of Frankia. There were two types of A and B mixed types except physiological type A and B, of which type B Nodules could be infected, nodular nodules could not be found in nodules, nodular nodules could be invaded by nodule A, nodular nodules could not be found in one of the two A and B mixed nodules, Type III also has type II; whole cell identification of most of the sugar xylose and galactose, a few strains contain only galactose or xylose, or contain galactose, xylose and arabinose. The G + C mol% in DNA is 65% ~ 74%.