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取聚丙烯膜(12×12cm、5丝厚)两张,其中一张将中央剪成2～3cm直径的孔,报纸(12×12cm)一张,胶圈两根。洗净罐头瓶壁、口,取有孔膜用胶圈封瓶口,再用报纸和膜在外第二次封套,即两膜夹一报纸的封口法。高压灭菌冷却后,无菌操作接种,接种时只揭掉报纸和外膜即可,再封时去掉报纸则成两膜封法。此法克服了瓶口大接种易污染的问题,同时又能清楚观察接种点萌发和表面污染情况,确保及时剔除和质量稳定,菌丝生长迅速,制种成功率大大提高。学生在教
Take polypropylene film (12 × 12cm, 5 wire thick) two, one of the central cut into 2 ~ 3cm diameter holes, newspaper (12 × 12cm) a rubber ring two. Wash the bottle wall cans, mouth, take a hole membrane with a rubber seal bottle, and then newspaper and film outside the second envelope, that is, a folder of two film clip a newspaper. After autoclaving and cooling, inoculation is aseptically operated, and only the newspaper and adventitia are removed when inoculation, and then the newspaper is sealed by two membrane sealing methods. This method overcomes bottleneck vaccination easy to pollute the problem, at the same time can clearly observe the germination and surface contamination at the point of inoculation to ensure timely culling and quality stability, rapid mycelial growth, seed production success rate greatly increased. Students are teaching