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目的 探讨载脂蛋白B基因 (apolipoproteinB ,apoB) 3’端可变数目串联重复序列 (variablenumbleoftandemre peats ,3’ -VNTR)多态与长沙地区脑血管病的关系。 方法 采用聚合酶链式反应 (PCR)结合聚丙烯酰胺凝胶电泳分析 13 0例脑梗死患者、13 0例脑出血患者、10 0例对照组人群apoB基因 3’ -VNTR多态 ;并采用氧化酶法测定血清甘油三脂 (TG)、总胆固醇 (TC)、高密度脂蛋白 (HDL)、低密度脂蛋白 (LDL) ,运用酶联免疫吸附法测定LP(a)浓度 ,免疫比浊法测定apoB -10 0及apoAI浓度。 结果 脑梗死组的apoB基因 3’ -VNTR的B等位基因频率 (0 .15 0 )高于对照组(0 .0 80 ) (P <0 .0 5 ) ;脑出血组apoB基因 3’ -VNTR的B等位基因频率 (0 .0 77)与对照组无显著差别。脑梗死组中 ,B/S或B/B基因型亚组的TC、TG、LDL -C水平明显高于S/S基因型 (P <0 .0 5 ) ;脑出血组中 ,B/S或B/B基因型亚组的各项血脂水平与S/S基因型无显著差别。 结论 1、长沙地区脑梗死可能与apoB基因 3’ -VNTR多态有关 ,而脑出血与之无关。 2、apoB基因 3’ -VNTR多态B等位基因可能通过影响血脂水平增加脑梗死易感性。
Objective To investigate the relationship between variable numbleoftandemre peats (3 ’-VNTR) polymorphisms at the 3’ end of apolipoprotein B (apoB) and cerebrovascular disease in Changsha area. Methods Polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis were used to analyze the 3 ’-VNTR polymorphism of apoB gene in 130 cerebral infarction patients, 130 cerebral hemorrhage patients and 100 control subjects. The oxidative Serum triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) were measured by enzymatic method. LP (a) concentration was measured by enzyme-linked immunosorbent assay ApoB -10 0 and apoAI concentrations were determined. Results The B allele frequency of apoB gene 3 ’-VNTR in cerebral infarction group (0.150) was higher than that in control group (0.080) (P <0.05) VNTR B allele frequency (0.077) and the control group no significant difference. In cerebral infarction group, the levels of TC, TG and LDL-C in B / S or B / B genotype subgroup were significantly higher than those in S / S genotype (P <0.05); in cerebral hemorrhage group, B / S Or B / B genotype sub-group of the various lipid levels and S / S genotype no significant difference. Conclusion 1, Changsha area cerebral infarction may be related to the apoB gene 3 ’-VNTR polymorphism, and cerebral hemorrhage has nothing to do with it. 2, apoB gene 3 ’-VNTR polymorphism B allele may affect the susceptibility of cerebral infarction by affecting blood lipid levels.