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采用循环伏安法和光谱法研究了羧甲基壳聚糖-Ag(CMC-Ag)配合物与鲱鱼精DNA之间的作用方式。结果发现,CMC-Ag配合物的存在导致Fe(CN)3-/4-6探针分子峰电流下降、式量电位正移,显示CMC-Ag和探针分子Fe(CN)3-/4-6与DNA之间存在竞争性作用,说明CMC-Ag通过插入方式与DNA相互作用;一定的扫描速率范围内,在CMC-Ag配合物参与的条件下,Fe(CN)3-/4-6在Au/DNA电极上的反应受吸附控制。CMC-Ag配合物使得鲱鱼精DNA的特征峰发生明显的减色效应,最大吸收峰峰位红移,进一步表明CMC-Ag配合物以插入方式与鲱鱼精DNA发生了相互作用,导致DNA分子的构象变化。
Cyclic voltammetry and spectroscopy were used to study the interaction between carboxymethyl chitosan-Ag (CMC-Ag) complexes and herring sperm DNA. The results showed that the peak current of Fe (CN) 3- / 4-6 probe decreased with the presence of CMC-Ag complex, and the mode potential was positive. It showed that CMC-Ag and probe molecule Fe (CN) 3- / 4 -6 and DNA, indicating that CMC-Ag interacts with DNA through insertion. Under the condition of CMC-Ag complex, the Fe (CN) 3- / 4- 6 The reaction on the Au / DNA electrode is controlled by adsorption. The CMC-Ag complex markedly reduced the characteristic peak of herring sperm DNA and the maximum absorption peak shifted red-shifted, indicating that CMC-Ag complex intercalate with herring sperm DNA by insertion, Conformational changes.