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测定乳酸脱氢酶(LD)同工酶常用电泳法。免疫沉淀法和柱层析法亦被用于测定急性心肌梗塞患者的LD_1活性。作者发现某些羟基化合物能特异地抑制LD 同工酶中骨骼肌型亚基(即M 亚基)。本文探索了用羟基化合物1,6一己二醇抑制M 亚基,测定血清LD_1和LD_1+LD_2活性的抑制分析法。作者将不同浓度的1,6一己二醇加到纯化的LD同工酶溶液内,经30℃保温15分钟,然后采用丙酮
Determination of lactate dehydrogenase (LD) isozymes commonly used electrophoresis. Immunoprecipitation and column chromatography were also used to determine LD 1 activity in patients with acute myocardial infarction. The authors found that certain hydroxy compounds specifically inhibit skeletal muscle subunits (ie, M subunits) in LD isozymes. This article explored the inhibition of M subunit with hydroxy compound 1,6-hexanediol and determination of the activity of serum LD_1 and LD_1 + LD_2. The authors added different concentrations of 1,6-hexanediol to the purified LD isozyme solution, incubated at 30 ° C for 15 minutes and then treated with acetone