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采用高效液相色谱法,以氢化考的松为内标,在Nova-PahC18柱上,甲醇-0.025mol/LKH2PO4水溶液为流动相,分离测定了洁肤霜中氯毒素和醋酸地塞米松的含量.操作简便,结果准确,测得氯霉素的回收率为98.61%,RSD=0.7%,地塞米松的回收率为97.65%,RSD=1.0%.
High performance liquid chromatography with hydrogenation of cortisone as an internal standard, Nova-PahC18 column, methanol-0.025mol / L KH2PO4 aqueous solution as the mobile phase separation and determination of cleansing cream chlorotoxin and dexamethasone acetate content. The results showed that the recovery of chloramphenicol was 98.61%, the RSD was 0.7%, the recovery of dexamethasone was 97.65% and the RSD was 1.0%.