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作者采用华枝睾吸虫石蜡包埋成虫切片做抗原片,应用酶联金葡菌 A 蛋白间接过氯化物酶试验(PPA—IIP)诊断华枝睾吸虫病.本试验测定病狗血清最适稀释度为1∶200.酶联金葡菌 A 蛋白(HRP—Protein A)适宜工作浓度为1∶40.诊断狗华枝睾吸虫病敏感性为94.4%,特异性为92.9%.比较纵横两种成虫切片对病狗血清的诊断价值,统计学上,两种成虫切片无显著差异(P>0.05).本法抗原性稳定,操作简便快速.
The authors used paraffin-embedded parasite-embedded adult worms as antigenic tablets and used enzyme-linked Staphylococcus aureus A protein indirect perchloralase assay (PPA-IIP) to diagnose schistosomiasis chinensis. This experiment determined the optimal dilution of diseased dog serum. The degree is 1:200. The suitable working concentration of enzyme-linked S. aureus A protein (HRP-Protein A) is 1:40. The diagnostic sensitivity of the test for Fascia schistosomiasis is 94.4% and the specificity is 92.9%. The diagnostic value of adult sera for diseased dog serum, statistically, there was no significant difference between the two adult worms (P>0.05). This method is stable antigenic and simple and fast.