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目的 观察鸡贫血病毒 ( chicken anem ia virus,CAV) vp3基因的体内抑瘤效应。方法 分别将 pc DNA- vp3(含 vp3基因的真核表达载体 )、pc DNA3 (空载体 )和小鼠腹水型肝癌细胞株 H2 2混合后 ,接种于 BAL B/ c小鼠皮下。几天后 ,处死动物 ,取肿瘤组织称重 ,比较试验组和对照组瘤重差异 ,确定 vp3基因的抑瘤效应。TU NEL 法鉴定 vp3在体内诱导肿瘤细胞死亡的方式。结果 注射 vp3基因的试验组肿瘤生长率明显低于注射空载体的对照组。TUNEL 试验的结果也表明 ,鸡贫血病毒 VP3蛋白在体内以凋亡方式诱导肿瘤细胞死亡。结论 预示 vp3基因可能在抑制肝癌细胞生长方面有一定的疗效
Objective To observe the antitumor effect of vp3 gene of chicken anemovirus (CAV) in vivo. Methods The pcDNA-vp3 (eukaryotic expression vector containing vp3 gene), pcDNA3 (empty vector) and mouse ascites hepatocellular carcinoma cell line H2 2 were mixed and subcutaneously in BALB / c mice. A few days later, the animals were sacrificed and the tumor tissue was weighed. The difference in tumor weight between the experimental group and the control group was compared to determine the anti-tumor effect of vp3 gene. TU NEL method to identify vp3 in vivo induction of tumor cell death. Results The tumor growth rate of the experimental group injected with vp3 gene was significantly lower than that of the control group injected with empty vector. The results of the TUNEL assay also showed that the chicken anemia virus VP3 protein induces tumor cell death in a apoptotic manner in vivo. The results suggest that vp3 gene may have a certain effect in inhibiting the growth of liver cancer cells