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目的:探讨中药芪蓝颗粒阻癌抑癌的作用机制。方法:SD大鼠150只,随机分为A、B、C、D、E共5组,A、B、C、D组用0.002%4NQO制备大鼠舌癌变模型,其中B、C、D组在制备模型的同时,用不同剂量芪蓝颗粒进行干预,A组为模型对照组,E组为正常对照组。各组于中药干预的9、18、27、36周时分别处死7只动物,取舌组织光镜下观察其病理变化,采用免疫组化技术检测各组表皮生长因子受体(EGFR)的表达。结果:A组总体癌变率39.29%,明显高于B、C、D组(P<0.05)。A组中EGFR的表达从正常口腔黏膜→各级异型增生→癌变,其阳性表达率呈递增趋势,差异有统计学意义(P<0.01)。在大鼠异常增生舌组织中A组的EGFR阳性表达率为6/11明显高于B、C、D组,差异有统计学意义(P<0.05)。在大鼠舌癌组织中进行中药干预组EGFR的阳性表达明显低于A组(P<0.05),差异有统计学意义(P<0.05)。E组EGFR蛋白表达均为阴性。结论:芪蓝颗粒具有明显的抑癌阻癌效果,它可能通过下调细胞中EGFR的表达水平,起到抑癌阻癌的作用。
Objective: To explore the mechanism of Qilan Granule in preventing and treating cancer. Methods: 150 Sprague-Dawley rats were randomly divided into 5 groups (A, B, C, D and E). The models of tongue carcinogenesis were made by 0.002% 4NQO in groups A, B, C and D, In the preparation of the model, with different doses of Qilan Granules intervention, A group model control group, E group as the normal control group. Seven rabbits were sacrificed at 9th, 18th, 27th, 36th weeks after intervention with Chinese herbs. Pathological changes of tongue tissue were observed under light microscope. The expression of epidermal growth factor receptor (EGFR) in each group was detected by immunohistochemistry . Results: The overall canceration rate of group A was 39.29%, which was significantly higher than that of group B, C and D (P <0.05). In group A, the expression of EGFR increased from normal oral mucosa → dysplasia at all levels to carcinogenesis (P <0.01). The positive rate of EGFR in group A was significantly higher than that in group B, C and D (P <0.05). The positive expression of EGFR in Chinese tongue cancer tissues was significantly lower than that in group A (P <0.05), the difference was statistically significant (P <0.05). E group EGFR protein expression were negative. Conclusion: Qilan Granule has obvious anti-cancer and anti-cancer effects. It may play a role of inhibiting cancer and blocking cancer by down-regulating the expression of EGFR in cells.