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目的:探讨甘草甜素对肾小球硬化模型中转化生长因子β1(TGFβ1)作用。方法:采用尾静脉注射阿霉素方法建立大鼠肾小球硬化模型。实验随机分为模型组、治疗组和对照组。检测各组第4和8周各项指标的变化。包括①24h尿蛋白定量,血尿素氮(BUN)、肌酐(Scr)、胆固醇(Cho)和白蛋白(Alb)。②各组肾皮质进行光镜等病理学检测。③应用免疫组织化学方法检测肾组织内转化生长因子β1(TGFβ1)蛋白质的表达。④采用标准曲线法进行荧光定量PCR检测肾组织内TGFβ1mRNA的表达。结果:①治疗组与模型组相比较,24h尿蛋白定量、BUN、Scr、Cho和Alb均有不同程度改善(p<0.05)。②肾皮质病理学改变肾小球硬化程度治疗组明显轻于模型组。③肾组织内TGFβ1蛋白和mRNA表达治疗组与模型组比较表达峰值均有不同程度降低(p<0.05)。结论:从蛋白和mRNA水平同时证实甘草甜素对TGFβ1蛋白和mRNA的表达有明显的抑制作用,对大鼠肾小球硬化有早期保护作用,为临床应用提供了依据。
Objective: To investigate the effect of glycyrrhizin on transforming growth factor β1 (TGFβ1) in glomerulosclerosis model. METHODS: Rat glomerular sclerosis model was established by tail vein injection of adriamycin. Experiments were randomly divided into model group, treatment group and control group. Changes in the indicators of the 4th and 8th weeks of each group were examined. Including 124h urinary protein quantification, blood urea nitrogen (BUN), creatinine (Scr), cholesterol (Cho) and albumin (Alb). 2 The renal cortex of each group was examined by light microscopy and other pathologies. 3 Immunohistochemical method was used to detect the expression of transforming growth factor-β1 (TGFβ1) protein in renal tissue. (4) Fluorescent quantitative PCR was used to detect the expression of TGFβ1 mRNA in renal tissue using standard curve method. Results: 1 Compared with the model group, the urinary protein quantitation, BUN, Scr, Cho and Alb in the treatment group were improved to different degrees (p<0.05). 2 renal cortical pathological changes in glomerular sclerosis treatment group was significantly lighter than the model group. 3 Compared with the model group, the expression of TGFβ1 protein and mRNA in renal tissue were decreased in different degrees (p<0.05). Conclusion: It is also confirmed from the level of protein and mRNA that glycyrrhizin has a significant inhibitory effect on the expression of TGFβ1 protein and mRNA, and has an early protective effect on glomerulosclerosis in rats, which provides a basis for clinical application.