甘草甜素对肾脏转化生长因子β1作用的实验研究

来源 :现代生物医学进展 | 被引量 : 0次 | 上传用户:houjz
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:探讨甘草甜素对肾小球硬化模型中转化生长因子β1(TGFβ1)作用。方法:采用尾静脉注射阿霉素方法建立大鼠肾小球硬化模型。实验随机分为模型组、治疗组和对照组。检测各组第4和8周各项指标的变化。包括①24h尿蛋白定量,血尿素氮(BUN)、肌酐(Scr)、胆固醇(Cho)和白蛋白(Alb)。②各组肾皮质进行光镜等病理学检测。③应用免疫组织化学方法检测肾组织内转化生长因子β1(TGFβ1)蛋白质的表达。④采用标准曲线法进行荧光定量PCR检测肾组织内TGFβ1mRNA的表达。结果:①治疗组与模型组相比较,24h尿蛋白定量、BUN、Scr、Cho和Alb均有不同程度改善(p<0.05)。②肾皮质病理学改变肾小球硬化程度治疗组明显轻于模型组。③肾组织内TGFβ1蛋白和mRNA表达治疗组与模型组比较表达峰值均有不同程度降低(p<0.05)。结论:从蛋白和mRNA水平同时证实甘草甜素对TGFβ1蛋白和mRNA的表达有明显的抑制作用,对大鼠肾小球硬化有早期保护作用,为临床应用提供了依据。 Objective: To investigate the effect of glycyrrhizin on transforming growth factor β1 (TGFβ1) in glomerulosclerosis model. METHODS: Rat glomerular sclerosis model was established by tail vein injection of adriamycin. Experiments were randomly divided into model group, treatment group and control group. Changes in the indicators of the 4th and 8th weeks of each group were examined. Including 124h urinary protein quantification, blood urea nitrogen (BUN), creatinine (Scr), cholesterol (Cho) and albumin (Alb). 2 The renal cortex of each group was examined by light microscopy and other pathologies. 3 Immunohistochemical method was used to detect the expression of transforming growth factor-β1 (TGFβ1) protein in renal tissue. (4) Fluorescent quantitative PCR was used to detect the expression of TGFβ1 mRNA in renal tissue using standard curve method. Results: 1 Compared with the model group, the urinary protein quantitation, BUN, Scr, Cho and Alb in the treatment group were improved to different degrees (p<0.05). 2 renal cortical pathological changes in glomerular sclerosis treatment group was significantly lighter than the model group. 3 Compared with the model group, the expression of TGFβ1 protein and mRNA in renal tissue were decreased in different degrees (p<0.05). Conclusion: It is also confirmed from the level of protein and mRNA that glycyrrhizin has a significant inhibitory effect on the expression of TGFβ1 protein and mRNA, and has an early protective effect on glomerulosclerosis in rats, which provides a basis for clinical application.
其他文献
为促进海上风电产业健康发展,鼓励优先开发优质资源,6月17日,发改委出台《关于海上风电上网电价政策的通知》,制定了海上风电上网电价。国家发改委在制定并出台《关于海上风
目的:研究小陷胸汤加味方对心肌缺血再灌注心肌的作用。方法:将32只健康家兔随机分为4组,假手术组(A组),缺血再灌注模型组(B组),小陷胸汤加味方低剂量组(C组),小陷胸汤加味方
“风在吼,马在叫,黄河在咆哮……”,一曲曲抗日战争时期创作的歌曲,回荡在首都大学生体育馆内,为纪念中国人民抗日战争胜利50周年而举办的驻京部队歌咏大会《热血长城》激荡
自磺胺、青霉素相继问世以来,抗生素的适用领域已从感染性疾病、某些癌症、延伸到许多非感染性的内外科疾病。笔者就有关抗生素适用领域的扩展介绍如下。 Since the advent
本文用示波极谱滴定法测定盐酸小檗碱及其制剂的含量。方法是:在醋酸盐缓冲溶液中(pH 4.3)盐酸小檗碱与过量四苯硼钠液反应,剩余Na-TPB液以硫酸亚铊滴定。测定结果:原料药的
本文对35例囊尾蚴病患者进行了间接非特异性和特异性肥大细胞脱颗粒试验。结果表明,此病患者的肥大细胞脱颗粒阳性率明显高于正常人,而且在囊尾蚴抗原作用下肥大细胞也可出现
据报道每天大剂量服用醋酸消旋α-生育酚(16000IU/日)可有效地阻止阿霉素引起的秃头症。现已进行了前瞻性试验以评定生育酚在防止由FAC疗法辅助化疗引起秃头症的效果。 25例
东日本学园大学的宫崎正三博士和城西大学的森本雍宪博士访华期间,应中国药学会上海分会的邀请,于1985年6月1日在沪作了学术报告。宫崎博士作了“医用高聚物在药剂上应用”
动物实验已证实人和鼠的α-降钙素基因调节肽(CGRP)能有效地抑制胃酸分泌和扩张血管,并可调节神经传递。人体实验也证实它是一种长效的皮肤血管扩张剂及胃酸分泌抑制剂。作
盐酸阿糖胞苷滴眼液的含量测定常采用提取后分光光度法、双波长分光光度法等,该法虽结果比较准确,但操作比较费时,又受仪器限制,不适应基层单位快速测定的需要。根据盐酸阿