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水稻种子贮存醇溶蛋白4a基因5′端上游区由两个启动子组成。将启动子Ⅰ与报告基因GUS融合，在根癌农杆菌介导下，转化烟草，经组织化学分析，确定启动子Ⅰ为种子特异性启动子。启动于Ⅰ的5′端系列缺失分析证明，－212～－167区段是启动子Ⅰ种子特异性关键调控元件。
The upstream of the 5 ’end of the gliadin 4a gene in rice seeds is composed of two promoters. The promoter I was fused with the GUS reporter gene and transformed into tobacco under Agrobacterium tumefaciens. The promoter I was determined to be a seed-specific promoter by histochemical analysis. The deletion analysis of the 5 ’end sequence of promoter I showed that the -212 ~ -167 segment is the key regulator of the seed Ⅰ of promoter Ⅰ.