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运用生物化小鼠抗DNP IgE抗体与抗生物素蛋白的复合体刺激嗜碱细胞性白血病(RBL-2H3)细胞,以对从细胞中游离的β-己糖胺酶的抑制作用为药理活性指标,对日本药典外生药收载的67种生药的水提取物(0.5g/ml)进行了研究。
The use of a biochemical mouse anti-DNP IgE antibody complex with avidin stimulates basophilic leukemia (RBL-2H3) cells to inhibit the release of β-hexosaminidase from cells as an indicator of pharmacological activity The water extracts (0.5 g/ml) of 67 crude drugs collected from exogenous drugs of the Japanese Pharmacopoeia were studied.