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目的:研究穿心莲内酯对外周血单核细胞表达IL-18相关细胞因子的影响。方法:不同浓度穿心莲内酯处理LPS刺激的PBMC(Peripheral Blood Mononuclear Cells,外周血单个核细胞)和LPS+IFNγ-/IL-4激活的磁珠分选PBM(PeripheralBlood Monocytes,外周血单核细胞),利用real-time RT-PCR法检测IL-18、IL-18BP、IL-18Rα、IL-18Rβ基因转录水平,ELISA法检测PBM上清中的IL-18、IL-18BP和IL-1β、IL-1Rα。结果:穿心莲内酯呈剂量和时间依赖地调节PBMC IL-18和IL-18BP的表达。穿心莲内酯处理使LPS刺激的PBMC IL-18BP转录增加,IL-18BP/IL-18比值升高;使LPS+IFNγ-激活的PBM表达IL-18BP/IL-18比值从9.60倍升高至214倍;IL-4激活的PBM表达IL-1Rα/IL-1β比值从9 200降低至6 520。结论:穿心莲内酯可调节激活的外周血单核细胞IL-18相关基因转录和表达,抑制炎症时IL-18的高表达。
Objective: To study the effect of andrographolide on peripheral blood mononuclear cells expressing IL-18 related cytokines. METHODS: Different concentrations of andrographolide were used to treat PBMC (Peripheral Blood Mononuclear Cells) stimulated by LPS and LPS+IFNγ-/IL-4 activated magnetic beads to sort PBM (Peripheral Blood Monocytes, peripheral blood mononuclear cells). The levels of IL-18, IL-18BP, IL-18Rα and IL-18Rβ genes were detected by real-time RT-PCR. IL-18, IL-18BP and IL-1β, IL in PBM supernatants were detected by ELISA. -1Rα. Results: Andrographolide dose- and time-dependently regulate the expression of PBMC IL-18 and IL-18BP. Andrographolide treatment increased the transcription of IL-18BP in PBMC stimulated by LPS and the ratio of IL-18BP/IL-18 increased. The ratio of IL-18BP/IL-18 in LPS+IFNγ-activated PBM increased from 9.60 to 214. Fold; IL-4 activated PBM expression IL-1Rα/IL-1β ratio decreased from 9 200 to 6 520. CONCLUSION: Andrographolide can regulate the transcription and expression of IL-18 related genes in activated peripheral blood mononuclear cells and inhibit the high expression of IL-18 in inflammation.