论文部分内容阅读
目的探讨纳米二氧化钛对人乳腺癌MCF-7细胞DNA损伤作用。方法不同粒径不同浓度的纳米二氧化钛对人乳腺癌MCF-7细胞作用,通过彗星实验检测细胞DNA损伤,评价遗传毒性效应。结果与对照组比较,30nm、50nm和100nm的纳米二氧化钛颗粒当浓度大于1μg/ml时均可对细胞产生遗传毒性效应,差异显著(P<0.05),且随着浓度的增加毒性作用增强。结论纳米Ti O2颗粒对人乳腺癌MCF-7细胞具有遗传毒性作用,且遗传毒性与纳米Ti O2颗粒浓度呈剂量依赖效应。
Objective To investigate the DNA damage effect of nano-TiO2 on human breast cancer MCF-7 cells. Methods The effect of nano-TiO2 with different particle size on human breast cancer MCF-7 cells was studied. The DNA damage was evaluated by comet assay and the genotoxic effect was evaluated. Results Compared with the control group, nano-titania particles of 30nm, 50nm and 100nm showed genotoxic effects on the cells when the concentration was higher than 1μg / ml (P <0.05), and the toxic effect was enhanced with the concentration increasing. Conclusion The nano-Ti O2 particles have a genotoxic effect on human breast cancer MCF-7 cells, and the genotoxicity is in a dose-dependent manner with the concentration of nano-Ti O2 particles.