材料与方法 菌株:供试菌种为NRRL2999寄生曲霉,它产生四种黄曲霉毒素:B_1、B_2、G_1、G_2。类黄酮化合物:木犀黄素圣草醇和5,7二羟基色酮。类黄酮化合物的混合物按花生壳中的比例(即:木犀黄素:圣草醇:5,7二羟基色酮为4:1:1的重量比)混合。试验浓度分别为0.01、0.02和0.06毫克/毫升。在培养好的寄生曲霉斜面培养基试管中,每管加入0.05％吐温20灭菌液10毫升,配成浓度为1×10~(10)(个孢子/毫升)的孢子悬浮液,然后在每个三角瓶内加入0.1毫升孢子悬浮液,置25℃定温培养。分别在4、7、11和14天后测定黄曲霉毒素的含量和菌丝体干重。 MATERIALS AND METHODS Strains: The test strain was NRRL2999 parasitic Aspergillus, which produced four aflatoxins: B_1, B_2, G_1, G_2. Flavonoids: Quercetin and 5,7-dihydroxychromone. The mixture of flavonoid compounds is mixed in proportions in the peanut hulls (ie, the weight ratio of ghrelin: saccharinate: 5,7 dihydroxychromone 4: 1: 1). The test concentrations were 0.01, 0.02 and 0.06 mg / ml, respectively. In cultured Aspergillus parasitic medium tube, 10 ml of 0.05% Tween 20 sterilization solution was added into each tube to prepare a spore suspension with a concentration of 1 × 10 ~ (10) (spores / ml) Add 0.1 ml spore suspension to each flask and incubate at 25 ° C. The aflatoxin content and mycelial dry weight were determined after 4, 7, 11 and 14 days, respectively.