论文部分内容阅读
目的建立祛脂胶囊中熊果酸的含量测定方法。方法采用高效液相色谱法,Waters(十八烷基硅烷键合硅胶柱250 mm×4.6 mm,5μm)。流动相:乙腈-0.5%磷酸溶液(72∶28),流速:1.0 ml/min,检测波长:205 nm,柱温:40℃。结果熊果酸进样量在0.22~2.2μg范围内线性良好(r=0.999 7),平均回收率102.3%,RSD=1.6%(n=6)。结论该方法简便、快速、准确,可用于该制剂的质量控制。
Objective To establish a method for the determination of ursolic acid in Quzhi Capsules. Methods High performance liquid chromatography (HPLC) was used. Waters (octadecylsilane bonded silica column 250 mm × 4.6 mm, 5 μm). Mobile phase: acetonitrile-0.5% phosphoric acid solution (72:28), flow rate: 1.0 ml / min, detection wavelength: 205 nm, column temperature: 40 ℃. Results The ursolic acid injection showed a good linearity (r = 0.999 7) within the range of 0.22-2.2 μg with an average recovery of 102.3% and RSD = 1.6% (n = 6). Conclusion The method is simple, rapid and accurate and can be used for the quality control of this preparation.