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目的了解昆明市游泳池水的致病菌污染状况。方法分别于2013、2014年4—10月,采集昆明市全部游泳池的水样,分离鉴定致病菌并进行药敏试验。结果共检测913件游泳池水样,检出致病菌28株,检出率为3.07%。假单胞菌(铜绿假单胞菌与恶臭假单胞菌)的检出率最高,为1.75%;其次是金黄色葡萄球菌,为0.98%;气单胞菌(嗜水气单胞菌与温和气单胞菌)的检出率最低,为0.33%。春季(4—5月)和秋季(9—10月)游泳池水中致病菌的检出率高于夏季(6—8月)。检出的9株金黄色葡萄球菌对青霉素G 100%耐药,且均带有肠毒素;检出的16株假单胞菌对头孢菌素、氨比西林、氨苄西林和阿莫西林-克拉维酸均100%耐药;检出的3株气单胞菌对头孢菌素、氨比西林、氨苄西林均100%耐药。结论昆明市区游泳池水检出多株多重耐药致病菌。
Objective To understand the pollution of pathogenic bacteria in swimming pool water in Kunming. Methods From April to October in 2013 and 2014, the water samples of all the swimming pools in Kunming were collected, and the pathogenic bacteria were separated and identified for drug susceptibility testing. Results A total of 913 swimming pool water samples were detected and 28 pathogenic bacteria were detected. The detection rate was 3.07%. Pseudomonas (Pseudomonas aeruginosa and Pseudomonas putida) the highest detection rate of 1.75%; followed by Staphylococcus aureus, was 0.98%; Aeromonas (Aeromonas hydrophila and Aeromonas sobria) the lowest detection rate was 0.33%. The detection rate of pathogenic bacteria in swimming pool water in spring (April-May) and autumn (September-October) was higher than that in summer (June-August). Nine strains of Staphylococcus aureus were detected, resistant to penicillin G 100%, all with enterotoxin. The 16 strains of Pseudomonas detected against cephalosporin, ampicillin, ampicillin and amoxicillin-carat All of the vitamins were 100% resistant. The three Aeromonas were 100% resistant to cephalosporin, ampicillin and ampicillin. Conclusion Multiple strains of multidrug-resistant bacteria were detected in swimming pool water in Kunming.