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【目的】研究浙江凤阳山黄山松阔叶混交林土壤理化性质以及微生物对碳源的利用状况,明确海拔变化对黄山松阔叶混交林林地土壤微生物功能多样性的影响。【方法】以黄山松在凤阳山的主要海拔分布范围(1 000~1 800 m)为准,选取中海拔(1 200 m)、中高海拔(1 500 m)、高海拔(1 800 m)3个海拔梯度,在每个海拔梯度的阳坡内选取排水较好、坡度较缓、长势适中的3个标准样地(20 m×20 m),采用Biolog Eco法研究该林分类型在不同海拔梯度下土壤微生物对碳源的利用特征,同时对土壤微生物利用各类碳源的特性进行非度量多维标度(NMDS)排序,进一步分析土壤微生物对各类碳源的利用情况。【结果】(1)在中高海拔处的土壤微生物活性最强,土壤微生物多样性指数随着海拔变化并无显著变化(P>0.05);(2)研究区内土壤微生物主要利用的碳源类型为碳水化合物(CH)、氨基酸类(AA)、聚合物类(PM),各海拔处土壤微生物对酚酸类(PA)和胺类(AM)碳源的利用率均较低,而对羧酸类(CA)碳源的利用存在显著差异;NMDS排序显示,随海拔变化,土壤微生物对6类碳源的利用出现显著差异(P<0.05);(3)土壤养分含量在中高海拔处最大,冗余分析显示土壤理化性质与土壤微生物对碳源的利用之间有较强的相关关系。【结论】中高海拔处土壤微生物对各类碳源的利用率都较高,土壤理化性质的改变是影响土壤微生物对碳源利用的重要因素。
【Objective】 The objective of this study was to investigate the soil physical and chemical properties of Pinus taiwanensis and broad-leaved mixed forest in Fengyang Mountain, Zhejiang Province, and the effect of microorganisms on the utilization of carbon sources. The effects of altitude on the microbial diversity in the mixed forest of Pinus taiwanensis and broad- 【Method】 Based on the main distribution range of Huangshan pine in Fengyang Mountain (1 000 ~ 1 800 m), mid-altitude (1 200 m), middle-high altitude (1 500 m) and high altitude (1 800 m) Three altitudes (20 m × 20 m) with good drainage, moderate slope and moderate elevation were selected in the sunny gradient of each elevation gradient using Biolog Eco method. At the same time, the non-metric multidimensional scaling (NMDS) was used to characterize the utilization of various types of carbon sources in soil microbes, and the utilization of various carbon sources by soil microorganisms was further analyzed. 【Result】 (1) The soil microbial activity was the strongest at middle and high altitude, while the diversity index of soil microbial diversity did not change significantly with elevation (P> 0.05). (2) The types of carbon sources that were mainly utilized by soil microorganisms in the study area For carbohydrates (CH), amino acids (AA), and polymers (PM), the utilization rate of soil microorganisms to phenolic acids (PA) and amines (AM) There were significant differences in the utilization of carbon sources in acid (CA); NMDS showed that soil microorganisms had significant differences in the utilization of 6 types of carbon sources (P <0.05) as the altitude changed; (3) Soil nutrient content was the highest at middle and high altitude , Redundancy analysis showed that there was a strong correlation between the soil physical and chemical properties and the utilization of carbon sources by soil microorganisms. 【Conclusion】 Soil microorganisms have high utilization rate of all kinds of carbon sources at middle and high altitude. The change of soil physical and chemical properties is an important factor affecting the utilization of soil carbon by soil microorganisms.