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目的建立HPLC-MS/MS同时检测大鼠血浆和组织中三聚氰胺和三聚氰酸含量的方法。方法以同位素标记的三聚氰胺和三聚氰酸作为内标物,大鼠血浆和组织经二氯甲烷-乙腈前处理后,经色谱柱分离,串联离子阱质谱采用ESI源按运行时间段切换正、负离子模式进行测定。结果在0.3～75μg/ml范围内,三聚氰酸和三聚氰胺具有良好的线性(r≥0.998)。在0.9、5.0、50μg/ml的给药大鼠的血浆样本中,三聚氰酸和三聚氰胺的回收率分别为97.4%～99.4%、96.8%～98.9%,两者的变异系数为2.43%～6.35%(日内)、1.70%～4.12%(日间)和0.30%～9.17%(日内)、0.75%～3.39%(日间);在0.9、5.0、50μg/ml的给药大鼠肝组织样本中,三聚氰酸和三聚氰胺的回收率分别为90.8%～104.6%、91.7%～106.0%,对应的变异系数为3.70%～5.14%(日内)、2.84%～4.50%(日间)和1.31%～2.21%(日内)、1.12%～3.36%(日间)。结论该方法样品前处理简单,基质干扰小,分析效率高,具有良好的精密度、准确度和特异性,是进行三聚氰酸、三聚氰胺在动物体内的组织代谢和毒性研究的理想分析方法。
OBJECTIVE To establish a method for the simultaneous determination of melamine and cyanuric acid in rat plasma and tissues by HPLC-MS / MS. Methods The isotope-labeled melamine and cyanuric acid were used as internal standard. The plasma and tissue of rats were pre-treated with methylene chloride-acetonitrile and separated by column. The ion trap mass spectrometry was switched by positive ion source (ESI) Negative ion mode. Results Cyanuric acid and melamine showed good linearity (r≥0.998) in the range of 0.3 ~ 75μg / ml. The recoveries of melamine and melamine in the plasma samples of 0.9, 5.0 and 50 μg / ml were 97.4% -99.4% and 96.8% -98.9%, respectively, with a coefficient of variation of 2.43% (Day), 0.70% ~ 4.12% (daytime), 0.30% ~ 9.17% (day) and 0.75% ~ 3.39% The recoveries of cyanuric acid and melamine in the sample ranged from 90.8% to 104.6% and from 91.7% to 106.0%, respectively, with corresponding coefficients of variation ranging from 3.70% to 5.14% (days), 2.84% to 4.50% (daytime) and 1.31% ~ 2.21% (days), 1.12% ~ 3.36% (daytime). Conclusions This method is simple and convenient for sample preparation with low matrix interference and high analytical efficiency. It has good precision, accuracy and specificity and is an ideal analytical method for studying the tissue metabolism and toxicity of cyanuric acid and melamine in animals.