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目的建立液相色谱-串联质谱同时测定人血浆中兰索拉唑及其代谢物兰索拉唑砜、5-羟基兰索拉唑浓度的方法。方法用Agilent SB-C18色谱柱,流动相为0.002 mol.L-1乙酸铵(用甲酸调pH为4)-乙腈(60∶40,v/v),流速为0.3 mL.min-1。用正离子电离,多离子反应监测进行定量分析。结果兰索拉唑、兰索拉唑砜、5-羟基兰索拉唑线性范围分别为5～3044,1.5～550,2～549.5 ng.mL-1,三者日内、日间精密度均小于10%,三者的提取回收率为81.55%～98.74%(RSD<10%)。结论本方法灵敏、准确、快速,可用于人血浆中兰索拉唑及其代谢物浓度的测定和药代动力学研究。
Objective To establish a method for the simultaneous determination of lansoprazole and its metabolites lansoprazole sulfone and 5-hydroxylansoprazole in human plasma by liquid chromatography-tandem mass spectrometry. Method The Agilent SB-C18 column was used and the mobile phase was 0.002 mol·L -1 ammonium acetate (adjusted to pH 4 with formic acid) -acetonitrile (60:40, v / v) at a flow rate of 0.3 mL · min -1. With positive ionization, multi-ion reaction monitoring for quantitative analysis. Results The linear ranges of lansoprazole, lansoprazole sulfone and 5-hydroxylansoprazole were 5 ~ 3044,1.5 ~ 550 and 2 ~ 549.5 ng.mL-1, respectively. The intra-day and inter-day precision were less than The recoveries of the three were 81.55% ~ 98.74% (RSD <10%). Conclusion The method is sensitive, accurate and rapid and can be used to determine the concentration and pharmacokinetics of lansoprazole and its metabolites in human plasma.