论文部分内容阅读
建立了测定熊胆中３种主要磷脂组分即磷脂酰胆碱（ＰＣ）、磷脂酰肌醇（ＰＩ）、磷脂酰乙醇胺（ＰＥ）的高效液相色谱法。将熊胆风干后研成粉末，经Ｖ（氯仿）ｖＶ（甲醇）＝ １ｖ１提取液处理后，在Ｐ－ＥＳｉｌｉｃａ柱上进行ＨＰＬＣ分析。流动相为Ｖ（乙腈）ｖＶ（甲醇）＝ ７６ｖ２４，流速１．５ ｍ Ｌ／ｍ ｉｎ，紫外检测波长２０５ ｎｍ 。ＰＣ的平均回收率为８９．３０％ ，相对标准差ＲＳＤ ＝ ２．０％ 。在精密度实验中，ＰＩ，ＰＣ，ＰＥ保留时间的ＲＳＤ 分别为３．９％ ，１．２％ ，１．９％ ，峰面积的ＲＳＤ分别为１．６％ ，０．８９％ ，２．６％ 。所建立的方法可作为熊胆中磷脂类化合物ＰＣ，ＰＩ，ＰＥ的一种简便、快速、准确的测定方法。
A high performance liquid chromatography (HPLC) was developed for the determination of phosphatidylcholine (PC), phosphatidylinositol (PI) and phosphatidylethanolamine (PE) The bear gall was air-dried and ground into powder. After V (chloroform) vV (methanol) = 1 v1 extract, HPLC analysis was performed on a P-ESilica column. The mobile phase was V (acetonitrile) vV (methanol) = 76v24, the flow rate was 1.5 m L / min and the UV detection wavelength was 205 nm. The average recovery rate of PC was 89.30%, relative standard deviation RSD = 2.0%. In the precision experiments, the RSDs of retention time of PI, PC and PE were 3.9%, 1.2% and 1.9%, respectively. The RSDs of peak areas were 1.6% and 0.89%, respectively. 6%. The established method can be used as a simple, rapid and accurate method for the determination of phospholipids PC, PI and PE in bile.