将丙烯酰化脯氨酸(acrylamide proline,AA-Pro)通过原子转移自由基聚合(ATRP)成功地固定到介孔二氧化硅上,构筑了一种新型色谱材料,并应用于糖肽的高效分离与富集。通过一系列测试方法证明丙烯酰化脯氨酸成功接枝在硅球上。同时,在糖肽分离富集测试中,该材料表现出良好的效果,从Fetuin酶解液中共获得15条糖肽,与富集前相比,检测到糖肽的个数增多,且信号强度增强。实验证明丙烯酰化脯氨酸修饰介孔二氧化硅可用于糖肽的分离富集中,同时为开发糖肽富集材料提供了一个新途径。 The acrylamide proline (AA-Pro) was successfully immobilized on mesoporous silica by atom transfer radical polymerization (ATRP) to form a new chromatographic material and was applied to the glycopeptide high efficiency Separation and enrichment. Acryloylproline was successfully grafted onto silica spheres through a series of test methods. At the same time, the material showed good effect in the separation and enrichment test of glycopeptide. A total of 15 glycopeptides were obtained from Fetuin hydrolyzate, and the number of glycopeptides detected was increased compared with that before enrichment, and the signal intensity Enhanced. Experiments show that acryloyl-proline modified mesoporous silica can be used for the separation and enrichment of glycopeptides and provide a new way for the development of glycopeptide-rich material.