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以鲨鱼软骨为原料,经盐酸胍抽提,丙酮分级沉淀,超滤,Sephadex G-75柱层折等步骤得到鲨鱼软骨制剂 1（SP1）和鲨鱼软骨制剂 2（SP2）。测定它们对肿瘤细胞和血管内皮细胞DNA合成的抑制效应,对小鼠移植瘤的抑癌作用,以及对小鼠脾脏和胸腺的影响。结果表明:SP1能同时抑制血管内皮细胞和直接抑制肿瘤细胞;SP2同时具有提高机体免疫功能、直控抑制肿瘤细胞和抑制血管内皮细胞的活性。整体实验说明它们都具有较强的抑制肿瘤生长的活性,对小鼠体重无明显影响,无明显毒副作用,显示了良好的应用前景。
Shark cartilage as the raw material, guanidine hydrochloride extraction, acetone precipitation, ultrafiltration, Sephadex G-75 column chromatography and other steps shark cartilage preparation 1 (SP1) and shark cartilage preparation 2 (SP2). The inhibitory effect on the DNA synthesis of tumor cells and vascular endothelial cells, the anti-tumor effect on the transplanted tumor in mice and the effect on the spleen and thymus of the mice were determined. The results showed that: SP1 can inhibit both vascular endothelial cells and tumor cells directly; SP2 also can improve the immune function, inhibit tumor cells and inhibit the activity of vascular endothelial cells. The overall experiment shows that they have strong inhibitory activity of tumor growth, no significant effect on body weight of mice, no obvious side effects, showing a good prospect.