论文部分内容阅读
目的 克隆并分析远源链球菌(S .sobrinus)可能与细胞分裂相关的新基因ftsK。方法采用“genomewalkingsystem(基因组步移系统)”克隆远源链球菌可能与细胞分裂相关的新基因ftsK ,与GenBank进行同源性分析,并用蛋白质分析软件分析ftsK的氨基酸序列。结果 所克隆的新基因由2 10 0个碱基组成,编码6 99个氨基酸。用GOR4软件对S .sobrinusftsK的二级结构预测,显示其主要由α螺旋和无规卷曲组成,另有小部分伸展线,但无β折叠,在其N末端有一跨膜区。对氨基酸序列进行保守区分析,氨基酸335～5 30序列具有ftsK SpoⅢE保守序列,同源性分析,除N末端2 2 6个氨基酸外,其余氨基酸序列与大肠杆菌细胞分裂蛋白FtsK的C末端2 3序列同源,2 17～6 35氨基酸与枯草杆菌SpoⅢE的C末端同源。结论 通过“基因组步移系统”克隆出的新基因可能与远源链球菌细胞分裂蛋白FtsK相关。
Objective To clone and analyze the novel gene ftsK related to cell division of Streptococcus sobrinus. METHODS: A novel gene ftsK, which may be related to cell division, was cloned by using “genomewalk system” and analyzed with GenBank. The amino acid sequence of ftsK was analyzed by protein analysis software. Results The cloned new gene consisted of 210 bases, encoding 699 amino acids. Secondary structure prediction of S. sobrinusftsK using GOR4 software showed that it predominantly consisted of alpha helix and random coil, with a minor stretch of extension but no beta sheet, with a transmembrane region at its N-terminus. The conserved regions of the amino acid sequences were analyzed. The amino acid sequences of 335 to 530 shared the conserved sequence of ftsK SpoIIIE. The amino acid sequence was identical to the C terminus of Escherichia coli FtsK, except for the N-terminal 266 amino acids Sequence homology, 2 17 ~ 6 35 amino acids and Bacillus subtilis Spo Ⅲ E C-terminal homology. Conclusion The new gene cloned by “genome walking system” may be related to FtsK, a far-sistant cell division protein of Streptococcus.