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采用改良的生物淘筛(Biopanning)流程,以人卵巢癌细胞为靶细胞进行5轮消减筛选,从噬菌体展示12肽文库(Ph.D-12phage displayed peptide library)筛选到靶向人卵巢癌的12肽克隆,通过ELISA、细胞免疫荧光法等方法从细胞水平鉴定了最佳阳性多肽噬菌体克隆R20靶向卵巢癌细胞的特异性和敏感性。结果显示:R20可以特异的、敏感的与卵巢癌细胞SKOV3结合,不与正常细胞或者其他癌细胞结合,具备进一步研发为卵巢癌导向分子元件而应用于卵巢癌靶向诊治试剂或药物研发的潜力。
Using a modified biopanning procedure, human ovarian cancer cells were selected as target cells for five rounds of screening. Ph.D-12phage displayed peptide library was screened for targeting human ovarian cancer 12 Peptide cloning, the specificity and sensitivity of the best positive peptide phage clone R20 targeting ovarian cancer cells were identified from the cellular level by ELISA, immunofluorescence and other methods. The results showed that R20 could specifically and sensitively bind to ovarian cancer cell line SKOV3 without binding to normal cells or other cancer cells and had the potential to be further developed as a targeted molecule for ovarian cancer for the targeted diagnosis and treatment of ovarian cancer. .