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目的探讨Z-VAD-FMK对外周刺激诱发的海马CA1区LTP的调制作用。方法20只雄性SD大鼠180-250 g,随机分为4组,单刺激坐骨神经诱发海马CA1区场电位。对照组强直刺激坐骨神经,诱发海马CA1区LTP。药物组在强直刺激前,于侧脑室微量注入大、中、小不同剂量的Z-VAD-FMK,10 min后强直刺激坐骨神经,诱导海马CA1区LTP,比较海马CA1区诱发场电位幅度在强直刺激前后的变化。结果①电刺激坐骨神经可在海马CA1区诱发出潜伏期固定,可重复出现的以负波为主的场电位;②对照组强直刺激后可出现持续时间2 h以上的LTP现象;③大、中、小剂量的Z-VAD-FMK抑制了强直刺激后海马CA1区LTP,主要表现在场电位幅度的变化,而且呈现剂量依赖性。结论LTP是学习与记忆的神经基础,Z-VAD-FMK能抑制海马LTP的诱导和形成且呈现剂量依赖性。
Objective To investigate the modulatory effect of Z-VAD-FMK on LTP in hippocampal CA1 region induced by peripheral stimulation. Methods Twenty male Sprague Dawley rats, 180-250 g, were randomly divided into 4 groups. Single sciatic nerve stimulation induced the field potentials in hippocampal CA1 region. In the control group, the sciatic nerve was stimulated with tetanic acid to induce LTP in hippocampal CA1 area. The rats in the drug group were injected with Z-VAD-FMK in large, medium and small doses at different doses before the tetanic stimulation. Ten minutes after the stimulation, the sciatic nerve was stimulated to induce LTP in the CA1 region of the hippocampus. Before and after the change. Results (1) Electrical stimulation of the sciatic nerve induced a fixed and repeatable negative field potential in the hippocampal CA1 region. ② In the control group, LTP with duration of more than 2 h appeared in the control group. (3) Small doses of Z-VAD-FMK inhibited the LTP of hippocampal CA1 region after tonic stimulation, mainly in the amplitude of field potential, and in a dose-dependent manner. Conclusion LTP is the neural basis for learning and memory. Z-VAD-FMK can inhibit the induction and formation of LTP in a dose-dependent manner.