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目的:研究黏附因子CD99及基质金属蛋白酶-2(MMP-2)在水泡状胎块和水肿性流产中的定位和表达及二者表达的相关性。方法:采用免疫组化SP法检测30例水肿性流产(HA)、20例完全性水泡状胎块(CHM)、20例部分性水泡状胎块(PHM)患者中CD99和MMP-2的表达水平。结果:①CD99主要表达于细胞滋养细胞胞膜,在HA、CHM和PHM中的表达率分别为49.83%、25.85%和25.40%;②MMP-2主要表达于合体滋养细胞胞质,在HA、CHM和PHM中的阳性表达率分别为36.7%、80.0%和85.0%;③CD99在HA组织中的表达率高于CHM和PHM组织,MMP-2在HA组织中的表达率低于CHM和PHM组织,且差异均有统计学意义(P<0.05);经Spearman相关性检验,CD99和MMP-2在三者中的表达率均呈负相关〔γ(HA)=-0.133,P<0.05;γ(CHM)=-0.154,P<0.05;γ(PHM)=-0.153,P<0.05〕。结论:CD99在水泡状胎块(HM)中表达下降,可能与其影响滋养细胞黏附及分化有关,MMP-2在HM中表达上升,可能与CD99表达下调,从而激活MMP-2表达,导致MMP-2过表达有关。二者均参与了HM的发生和发展,且CD99表达的下降在一定程度上促进了MMP-2的表达,从而为HM的早期诊断和临床治疗提供了依据。
Objective: To study the localization and expression of adhesion molecules CD99 and matrix metalloproteinase-2 (MMP-2) in the blister-shaped blocks and edematous abortion. Methods: The expressions of CD99 and MMP-2 in 30 cases of edematous abortion (HA), 20 cases of complete blister (CHM) and 20 cases of partial vesicular motility (PHM) were detected by immunohistochemical SP method Level. The expression of CD99 was mainly in the cytotrophoblast cell membrane, the expression rates of which were 49.83%, 25.85% and 25.40% respectively in HA, CHM and PHM; ②MMP-2 was mainly expressed in the cytoplasm of syncytiotrophoblast, The positive expression rate of PH99 in PHM was 36.7%, 80.0% and 85.0%, respectively. ③The expression of CD99 in HA tissue was higher than that in CHM and PHM tissues, while the expression rate of MMP-2 in HA tissue was lower than that in CHM and PHM tissues (P <0.05). The Spearman correlation test showed that the expression rates of CD99 and MMP-2 were negatively correlated (γ (HA) = - 0.133, P < ) = - 0.154, P <0.05; γ (PHM) = - 0.153, P <0.05]. CONCLUSION: The decreased expression of CD99 in vesicular mothers (HM) may be related to its influence on the adhesion and differentiation of trophoblast cells. The expression of MMP-2 may be related to the upregulation of MMP-2 in HM and downregulation of CD99 expression, resulting in activation of MMP- 2 overexpression related. Both of them are involved in the occurrence and development of HM, and the decrease of CD99 expression promotes the expression of MMP-2 to a certain extent, which provides a basis for the early diagnosis and clinical treatment of HM.