【摘 要】
：
猪精液中含有高浓度的蛋白质,因此提取高纯度DNA较为困难;试验分别用水煮法、异硫氰酸胍法和Trizol法三种方法对连续稀释梯度猪精液提取猪细小病毒DNA,应用Taqman荧光定量PCR
【基金项目】
：
河南省杰出科研人才创新工程项目;
论文部分内容阅读
猪精液中含有高浓度的蛋白质,因此提取高纯度DNA较为困难;试验分别用水煮法、异硫氰酸胍法和Trizol法三种方法对连续稀释梯度猪精液提取猪细小病毒DNA,应用Taqman荧光定量PCR技术评价提取效果并加以对比.结果发现Trizol法可以提取出高纯度的DNA,但因Trizol试剂价格昂贵,不适用临床检测;比较另外两种方法,水煮法较异硫氰酸胍抽提法更为有效,是一种迅速、简便、经济、高效的DNA提取方法,适合于临床大量样品的检测.
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