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用核磁共振(NMR)方法研究了中药山莨菪碱(anisodamine)与结构不同的3种磷脂脂质体相互作用,二棕榈酰磷脂酰胆碱(DPPC)极性基团空阻较大,山莨菪碱三级胺端不能与P-O键作用,仍处于水相,苯环只能嵌入到甘油骨架C-2附近。二棕榈酰磷脂酸(DPPA)极性头空间位阻较小,山莨菪碱苯环可以直接插入到靠近脂酰链γ-次甲基的位置,而三级胺端与极性头发生静电作用,并且药物可以提高DPPA脂质体的流动性。山莨菪碱通过三级胺端与鞘磷脂(SPM)极性头静电作用较强,而苯环位于SPM脂双层亲水和疏水区界面。药物对3种磷脂双层结构影响很小。
The interaction between anisodamine and three phospholipid liposomes with different structures was studied by nuclear magnetic resonance (NMR) method. The dipoles of phosphatidylcholine (DPPC) The tertiary amine end can not function with the PO bond, is still in the aqueous phase, and the benzene ring can only be embedded near the glycerol backbone C-2. Dipolarityl diphosphatidic acid (DPPA) polar head space steric hindrance, anisodamine benzene ring can be directly inserted close to the acyl chain γ-methine position, while the tertiary amine side and the polar head electrostatic effect , And drugs can improve the fluidity of DPPA liposomes. Anisodamine strongly interacts with the sphingomyelin (SPM) polar head via the tertiary amine side, whereas the benzene ring is located at the hydrophilic and hydrophobic interface of the SPM lipid bilayer. Drugs have little effect on the bilayer structure of the three phospholipids.