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目的探讨工作场所空气中氨的全自动流动注射化学分析方法。方法工作场所空气中的氨被稀核酸溶液吸收后,用多通道全自动流动注射分析仪,在二水亚硝基铁氰化钠的存在下,溶液中的氨离子与水杨酸钠和次氯酸盐在碱性磷酸盐缓冲液中加热后,显色反应生成翡翠绿颜色(二水亚硝基铁氰化钠增强颜色),在510 nm的波长下被检测,电信号(峰面积)与吸收液中铵的浓度存在线性关系,可检测工作场所中的氨含量。结果在浓度为0.10~5.00 mg/L范围内呈线性关系,r≥0.999 9,方法检出限为0.0017 mg/m~3。相对标准偏差为1.60%~2.42%,加标回收率100.0%~119.0%。结论该方法操作简便、灵敏度高、检出限低、检测时间短,适用于实验室大批量低浓度样品的检测。
Objective To investigate the method of automatic flow injection chemical analysis of ammonia in workplace air. Methods Ammonia in the air of the workplace was absorbed by the dilute nucleic acid solution, and then a multi-channel automatic flow injection analyzer was used. In the presence of two nitrosylated sodium cyanide solution, the ammonia ion in the solution reacted with sodium salicylate and Chlorate heated in alkaline phosphate buffer, the color reaction emerald green color (nitrite sodium nitrite enhanced color), was detected at 510 nm wavelength, the electrical signal (peak area) There is a linear relationship between the concentration of ammonium in the absorption solution and the ammonia content in the workplace. The results showed a linear relationship in the concentration range of 0.10-5.00 mg / L, r ≥ 0.9999, and the detection limit was 0.0017 mg / m ~ 3. The relative standard deviations were 1.60% ~ 2.42%, and the spiked recoveries ranged from 100.0% to 119.0%. Conclusion The method is simple and easy to operate with high sensitivity, low detection limit and short detection time. It is suitable for the detection of high-concentration and low-concentration samples in laboratories.