利用自由基沉淀聚合反应合成了不同组成的、具有可逆溶解 -沉淀性能的甲基丙烯酸 -苯乙烯 -顺丁烯二酸酐三元共聚物载体 ,并测定了其临界 pH值 (2 .4 7～ 3.5 1) .利用载体上的酸酐基团固定葡萄糖淀粉酶 ,得到了具有液相酶和固相酶两者优点的固定化酶 .考察了单体组成、固定化反应时间对固定化酶酶活的影响和可回收利用性 .固定化酶 (湿 )酶活可达 2 347u/ g ,经溶解 -沉淀循环 3次回收后的固定化酶酶活可保留 73% . The terpolymers of methacrylic acid-styrene-maleic anhydride with different compositions and reversible dissolution-precipitation behavior were synthesized by free-radical precipitation polymerization. The critical pH (2-4.7 ~ 3.5 1) Immobilized glucoamylase was immobilized on the carrier, and the immobilized enzyme with the advantages of liquid phase enzyme and solid phase enzyme was obtained. The effect of monomer composition and immobilization reaction time on the activity of immobilized enzyme Of immobilized enzyme (wet) enzyme activity up to 2 347u / g, after the dissolution - precipitation cycle recovery of three fixed enzyme activity can retain 73%.