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蔗糖磷酸合成酶是调节植物蔗糖合成的关键酶之一。本研究根据该段序列上预测的顺式作用元件将SPSⅢ5’侧翼序列不同长度的片段与GUS基因融合,成功构建了3个缺失表达载体,为进一步的甘蔗SPSⅢ启动子活性区域鉴定提供基础。
Sucrose phosphate synthase is one of the key enzymes that regulate plant sucrose synthesis. According to the predicted cis-acting element in this sequence, three fragments with different lengths of flanking sequence of 5 ’flanking sequence were fused with GUS gene. Three deletion expression vectors were successfully constructed, which provided the basis for the further identification of SPSⅢ promoter region.