通常胎儿三体的产前诊断,是通过羊水的细胞遗传学分析作出的。然而,这种方法需要很长的实验室程序,费用昂贵,不适于作大规模的产前筛选。荧光聚合酶链反应(PCR)则是一种快速、廉价、适用、甚至可以单一胎儿细胞作出三体诊断的方法。本文报道了用荧光PCR和两种多形态STRs(D13S258和D13S631),在羊水中快速作出13三体产前诊断。 Prenatal diagnosis of the trisomy is usually made by cytogenetic analysis of amniotic fluid. However, this method requires very long laboratory procedures and is expensive and not suitable for large-scale prenatal screening. Fluorescent polymerase chain reaction (PCR) is a fast, inexpensive, affordable and even trisomic method that allows for single fetal cells. This paper reports the rapid diagnosis of trisomy 13 prenatal diagnosis in amniotic fluid using fluorescent PCR and two polymorphic STRs (D13S258 and D13S631).