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目的:对双丹口服液(Shuang Dan oral liquid,SD)的入血成分进行分析研究,进而探讨其药效物质基础。方法:采用血清药物化学的研究方法,建立双丹冻干粉经口灌胃后大鼠血清的HPLC指纹图谱,结合体外样品指纹图谱,分析比较双丹冻干粉及单味药材给药后所得血清样品,鉴定双丹冻干粉大鼠血中移行成分。结果:通过与文献中标准物质的保留时间确定大鼠口服双丹冻干粉后有8种血清移行原型成分,分别为源自牡丹皮的没食子酸、芍药苷和丹皮酚,源自丹参的丹参素、原儿茶酸、咖啡酸、迷迭香酸和丹酚酸B,此8种成分将有可能成为双丹方药效作用的物质基础。结论:本实验初步确定了双丹口服液的入血成分,为后续双丹方体内直接作用物质的确定和药效相关性研究提供了数据支持,并为其药效物质基础的阐明奠定了理论基础。
OBJECTIVE: To analyze the blood components of Shuang Dan oral liquid (SD), and to explore the basis of its pharmacodynamic substance. Methods: Serum HPLC method was used to establish the HPLC fingerprint of serum of Shuangdan freeze-dried powder after oral gavage. The fingerprint of Shuangdan freeze-dried powder and single herbs were analyzed and compared with the fingerprint of in vitro samples Serum samples were identified Shuandam freeze-dried powder in rats with blood migration components. Results: Eight kinds of serum migrating prototype components were determined after oral administration of Shuangdan freeze-dried powder in rats, which were respectively obtained from the paeoniflorin, paeoniflorin and paeonol, Danshensu, protocatechuic acid, caffeic acid, rosmarinic acid and salvianolic acid B, the eight components will likely become the material basis for the double Dan Fang pharmacodynamic effect. Conclusion: The preliminary determination of the double-Dan oral liquid into the blood components, follow-up for the determination of direct effect of the substance and efficacy of double-Danshen provide data to support and lay the foundation for the elucidation of the material basis of its efficacy basis.