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目的:探讨慢性淋巴细胞白血病(CLL)患者外周血CD4+CD25+调节性T细胞(Treg细胞)表达及其与预后的相关性。方法:采用流式细胞仪检测50例健康者(对照组)及30例CLL患者(CLL组)治疗前后CD4+CD25+Treg、CD4+CD25+Foxp3+Treg水平。结果:初诊CLL组患者外周血T淋巴细胞数量、CD4+CD25+Treg、CD4+CD25+Foxp3+Treg水平均显著高于对照组,差异有统计学意义(P<0.05)。治疗后,CLL组患者外周血T淋巴细胞数量、CD4+CD25+Foxp3+Treg水平显著降低,与初诊时比较差异有统计学意义(P<0.05),但仍高于对照组(P<0.05)。CLL患者中,Binet临床分期A期患者CD4+CD25+Foxp3+Treg水平显著低于B/C期患者,差异有统计学意义(P<0.05)。Spearman相关分析结果显示,Binet临床分期与CD4+CD25+Foxp3+Treg水平呈正相关(r=0.511,P<0.05)。结论:CD4+CD25+Foxp3+Treg水平可能是评估CLL患者预后的有效指标。
Objective: To investigate the expression of CD4 + CD25 + regulatory T cells (Tregs) in peripheral blood of patients with chronic lymphocytic leukemia (CLL) and its relationship with prognosis. Methods: The levels of CD4 + CD25 + Treg, CD4 + CD25 + Foxp3 + Treg in 50 healthy people (control group) and 30 CLL patients (CLL group) before and after treatment were detected by flow cytometry. Results: The numbers of T lymphocytes, the levels of CD4 + CD25 + Treg, CD4 + CD25 + Foxp3 + Treg in the newly diagnosed CLL group were significantly higher than those in the control group (P <0.05). After treatment, the number of T lymphocytes and the level of CD4 + CD25 + Foxp3 + Treg in peripheral blood of CLL group were significantly lower than those of the control group (P <0.05), but still higher than those of the control group (P <0.05) . Among patients with CLL, the level of CD4 + CD25 + Foxp3 + Treg in patients with stage B clinical stage Binet was significantly lower than that in stage B / C patients (P <0.05). Spearman correlation analysis showed that the clinical stage of Binet was positively correlated with the level of CD4 + CD25 + Foxp3 + Treg (r = 0.511, P <0.05). Conclusion: The level of CD4 + CD25 + Foxp3 + Treg may be a useful indicator to evaluate the prognosis of patients with CLL.