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在Prasad等报告了锌缺乏的病人发育不良和第二性征迟缓后,人体对锌的需要受到重视。血清锌浓度的降低指示几种疾病,锌盐被成功地用于治疗肠原性肢端皮炎和加速伤口愈合。测定血清锌浓度有很多方法,如比色法;萤光法;原子吸收分光光度法(AAS);阳极脱压法(anodic stripping voltammetry);X射线萤光法和中子活化分析。虽然AAS被认为是测定锌的参照法,但它需要昂贵的设备。比色法是临床化学中的普通方法,但如用二苯硫卡巴腙法等操作也嫌繁杂,且为了除去血清中共存的重金属干扰还要用危险的氰化物。近来Fujii等报告了一个使用1-(2-吡啶重氮)α-萘酚(PAN)和界面活性剂测定血清锌的直接比色法。作者提出的一个使用α-(α-吡啶重
After Prasad et al. Reported zinc deficiencies in patients with stunting and secondary sexual retardation, the body’s zinc needs attention. The decrease in serum zinc concentrations is indicative of several diseases and zinc salts have been successfully used to treat enterocolitical dermatitis and accelerate wound healing. There are many methods of determining serum zinc concentration, such as colorimetric; fluorescence; AAS; anodic stripping voltammetry; X-ray fluorescence and neutron activation analysis. Although AAS is considered a reference method for the determination of zinc, it requires expensive equipment. Colorimetric method is a common method in clinical chemistry, but such as using diphenylsulfocarbazone method is also too complicated to operate, and in order to remove the interference of heavy metals in serum also use dangerous cyanide. Recently, Fujii et al. Reported a direct colorimetric method for the determination of serum zinc using 1- (2-pyridine diazo) α-naphthol (PAN) and surfactants. The authors propose a use of α- (α-pyridine weight