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甲烷氧化菌素(methanobactin,mb)是具有过氧化氢还原酶活性的荧光肽.从甲基弯菌Methylosinus trichospo-rium IMV3011限铜培养介质中分离mb,采用紫外可见全波长扫描法观察mb催化对苯二酚还原氯金酸合成纳米金的作用和影响,当mb/氯金酸/对苯二酚反应液中mb的浓度分别是2.5×10-5mol/L、5.0×10-5mol/L和1.0×10-4mol/L时,形成的纳米金溶液的特征峰分别是561.5 nm(OD561=0.158)、548.0 nm(OD5 48=0.426)、536.5 nm(OD5 36=0.541),特征峰波长减小,对应的吸光值增大,表明mb能够催化对苯二酚还原氯金酸合成纳米金,并且可以通过调控mb的浓度控制纳米金的合成量及粒径大小.
Methanobactin (mb) is a fluorescent peptide with hydrogen peroxide reductase activity.Mb was isolated from the limiting copper culture medium of Methylosinus trichospo-rium IMV3011 and observed by UV-visible full-wavelength scanning When the concentrations of mb in mb / chloroauric acid / hydroquinone reaction solution were 2.5 × 10-5mol / L, 5.0 × 10-5mol / L and 1.0 × 10-4mol / L, the characteristic peaks of the gold nanocrystals formed were 561.5 nm (OD561 = 0.158), 548.0 nm (OD5 48 = 0.426) and 536.5 nm (OD5 36 = 0.541) , Corresponding to the increase of absorbance, indicating that mb can catalyze the hydroquinone reduction of chlorauronic acid synthesis of gold nanoparticles, and can control the concentration of mb nano-gold synthesis and particle size.