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白僵菌是桑天牛的生物防治菌种之一,探究白僵菌菌种退化的遗传机理有助于利用生物工程技术控制菌种退化,提高其生防效率。利用RAPD技术分析第1、6、12、14、16、18和20代白僵菌菌株在基因水平的变异情况,结果表明各代菌株间既有共有的条带,同时又有谱带的增加或减少,与第1、6代菌株相比,第12代菌株的基因组DNA发生了明显的变化,第14代时继续变化,之后的第16、18和20代菌株又趋于稳定。供试的7代菌株间的遗传距离变化范围在0.05～0.60之间,存在较明显的遗传差异,且随传代次数增加,菌株的菌丝生长量增大,产孢能力降低和对桑天牛幼虫致病能力降低等检测结果具有一定的一致性。研究结果提示,可利用RAPD技术进行桑天牛幼虫致病性白僵菌菌株退化的早期遗传检测。
Beauveria bassiana is one of the biological control species of Morus alba, exploring the genetic mechanism of the degradation of Beauveria bassiana helps to control the degradation of the species by bioengineering technology and improve its bio-control efficiency. The variation of Beauveria bassiana isolates on the 1st, 6th, 12th, 14th, 16th, 18th and 20th generations at the genetic level was analyzed by RAPD. The results showed that there was a common band between the isolates at the same time, Compared with the first and the sixth generation strains, the genomic DNA of the 12th generation strain had obvious changes, and continued to change on the 14th generation. Strain 16, 18 and 20 strains tended to be stable again afterwards. The genetic distance between the 7 generations of the tested strains ranged from 0.05 to 0.60, and there was obvious genetic difference. With the increase of passage times, the mycelial growth of the strains increased, the spore forming ability decreased, Larvae reduced pathogenicity and other test results have a certain consistency. The results suggest that the RAPD technique can be used to carry out the early genetic test for the degeneration of pathogenic Beauveria bassiana strains.