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Pf155/RESA.是一种分子量为155kDa耐热的可溶性抗原。该抗原与裂殖子的微线体和棒状体有关,当裂殖子入侵红细胞时沉集于膜上。体外培养恶性疟原虫,Pf155/RESA则释放到培养基中。用恶性疟原虫的坦桑尼亚株进行体外培养,离心收集培养24h后的上清。经免疫吸附柱吸附后洗脱,得到吸附的蛋白,其中包括Pf155/RESA及其相关抗原。每毫升内1～4μg这种蛋白即可抑制环状体感染红细胞表面的免疫荧光。洗脱的蛋白用SDS-PAGE和免疫印渍法分析。结果表明,在分子量为10～250kDa间至少有20种多肽,在有抗原
Pf155 / RESA. Is a soluble antigen with a molecular weight of 155kDa. This antigen is associated with the merozoites and rods of the merozoites that sink to the membrane when the merozoites invade the erythrocytes. Plasmodium falciparum was cultured in vitro and Pf155 / RESA was released into the medium. The tanzania strain of Plasmodium falciparum was used for in vitro culture, and the supernatant after culturing for 24 hours was collected by centrifugation. Adsorbed after adsorption column eluted to obtain adsorbed proteins, including Pf155 / RESA and its associated antigen. 1 ~ 4μg per milliliter of this protein can inhibit the ring-shaped infection of erythrocyte surface immunofluorescence. The eluted protein was analyzed by SDS-PAGE and immunoblotting. The results showed that in the molecular weight of 10 ~ 250kDa at least 20 kinds of peptides, in the presence of antigen