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培养在附加不同种类的激素和不同激素浓度组合的 MS 基本培养基上的杜仲下胚轴切段,能诱导出愈伤组织,但诱导出的愈伤组织的生物产量及芽数不同:1.含0.2mgL~(-1)～2mgL(-1)BA 和2mgL(-1)ZT的 MS 培养基上,外植体诱导出了愈伤组织和芽,而在附加2mgL(-1)KT 的 MS 培养基上,外植体只能诱导出愈伤组织。2.在 MS+8mgL(-1)BA 的培养基上,只有愈伤组织的产生而无芽的分化,说明8mgL(-1)BA 抑制了芽的发生。3.MS+2mgL(-1)BA+0.2mgL(-1)生长素(NAA、IAA、IBA)的培养基,能促进外植体诱导出愈伤组织,也能分化出芽。
Culturing in the hypocotyl cuttings of MS medium supplemented with different kinds of hormones and combinations of different hormone concentrations induced callus, but the callus induced was different in callus production and shoot number. The explants induced callus and shoots on MS medium containing 0.2 mg L -1 -1 2 mg L -1 BA and 2 mg L -1 ZT, MS medium, explants can only induce callus. On the medium of MS + 8mgL (-1) BA, only callus was produced without bud differentiation, indicating that 8mgL (-1) BA inhibited the bud formation. The medium of MS + 2mgL (-1) BA + 0.2mgL (-1) auxin (NAA, IAA, IBA) can promote the explants to induce callus and also differentiate into buds.