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目的测定秦皮甲素和秦皮乙素在大鼠体内的血药浓度及药代动力学参数。方法大鼠口服给予秦皮甲素和秦皮乙素各60 mg·kg~(-1),于给药后不同时间点采血,UPLC法测定血浆秦皮甲素和秦皮乙素含量,3P87计算药代动力学参数。结果秦皮甲素和秦皮乙素的主要药动学参数:T_(max)分别为0.164,0.0643 h;C_(max)分别为4.778,3.576μg·m L~(-1);AUC_(0-4)分别为5.360,5.682μg·m L~(-1)·h;AUC_(0-∞)分别为7.449,11.691μg·m L~(-1)·h;CL/F(s)分别为7.938,11.055 mg·kg~(-1)·h·μg~(-1)·m L;二者均符合二室模型。结论建立了大鼠血浆中秦皮甲素和秦皮乙素的UPLC检测方法,方法灵敏准确,适用于药代动力学研究。
Objective To determine the plasma concentration and pharmacokinetic parameters of aesculin and aesculetin in rats. Methods Aesculin and aesculetin were respectively administrated 60 mg · kg ~ (-1) at different time points after administration. The contents of aesculin and aesculetin were determined by UPLC and the pharmacokinetics were calculated by 3P87 Learning parameters. Results The main pharmacokinetic parameters of aesculin and aesculetin were: T max (max) 0.164 and 0.0643 h, C max 4.778 and 3.576 μg · m L -1, AUC 0-4 ) Were 5.360 and 5.682 μg · m L -1 · h, respectively; AUC 0 -∞ were 7.449 and 11.691 μg · m L -1 · h, respectively, and CL / Fs were 7.938 , 11.055 mg · kg -1 · h · μg -1 · m L, respectively. Conclusion UPLC detection method of aesculin and aesculetin in rat plasma was established. The method is sensitive and accurate and suitable for pharmacokinetic studies.