多肽类物质对生物体生理机能的调节研究受到研究者越来越多的重视,分离技术更是其技术实现产业化的关键,高效液相色谱法分离多肽类物质具有分离效果好、速度快、样品容量大、回收率高的特点,是目前生物多肽的主要分离纯化方法。文章综述了高效液相色谱法分离多肽类物质的原理、特点及应用。 Research on regulation of physiological function of living organisms by polypeptide has been paid more and more attention by researchers. Separation technology is the key to industrialization of its technology. High performance liquid chromatography (HPLC) separation of peptides has good separation effect, Large sample size, high recovery rate, is currently the main separation and purification of biological peptides. The article summarizes the principles, characteristics and applications of HPLC separation of peptides.