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AIM To evaluate antihepatoma effect ofantisense phosphorothioate oligodeo-xyribonucleotides(S-ODNs)targeted to alpha-fetoprotein(AFP)genes in vitro and in nudemice.METHODS AFP gene expression was examinedby immunocytochemical method or enzyme-linked immunosorbent assay.Effect of S-ODNson SMMC-7721 human hepatoma cell growth invitro was determined using microculturetetrazolium assay.In vivo antitumor activitiesof S-ODNs were monitored by measuring tumorweight differences in treated and control micebearing SMMC-7721 xenografts.Induction of cellapoptosis was evaluated by fluorescence-activated cell sorter(FACS)analysis.RESULTS Antisense S-ODN treatment led toreduced AFP gene expression.Specificantisense S-ODNs,but not control S-ODNs,inhibited the growth of heaptoma cells in vitro.In vivo,only antisense S-ODNs exhibitedobvious antitumor activities.FACS analysisrevealed that the growth inhibition by antisenseS-ODNs was associated with their cell apoptosisinduction.CONCLUSION Antisense S-ODNs targeted toAFP genes inhibit the growth of human hepatomacells and solid hepatoma,which is related totheir cell apoptosis induction.
AIM To evaluate antihepatoma effect of antisense phosphorothioate oligodeo-xyribonucleotides(S-ODNs)targeted to alpha-fetoprotein(AFP)genes in vitro and in nudemice.METHODS AFP gene expression was examined by immunocytochemical method or enzyme-linked immunosorbent assay.Effect of S-ODNson SMMC-7721 human hepatoma cell growth invitro was determined using microculturetetrazolium assay.In vivo antitumor activitiesof S-ODNs were monitored by measuring tumorweight differences in treated and control micebearing SMMC-7721 xenografts.Induction of cellapoptosis was evaluated by fluorescence-activated cell sorter(FACS )analysis.RESULTS Antisense S-ODN treatment led toreduced AFP gene expression.Specificantisense S-ODNs,but not control S-ODNs,inhibited the growth of heaptoma cells in vitro.In vivo,only antisense S-ODNs exhibitedobvious antitumor activities.FACS analysisrevealed That the growth inhibition by antisenseS-ODNs was associated with their cell apoptosisinduction.CONCLUSION Antisense S-ODN s targeted toAFP genes inhibit the growth of human hepatomacells and solid hepatoma,which is related totheir cell apoptosis induction.