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目前 ,利用重质原油(19°API重度 )和伴生水来研究微生物生长对于水的粘度、pH值、油水界面张力的影响 ,关于原油采收率从水驱残余油来进行驱替实验 ,研究营养物的浓度、孵化温度和碳酸盐含量的影响。研究结果表明 ,利用糖蜜作为基本的营养物可以将在地层水和原油中的本源微生物种群激活 ,而产生生物表面活性剂、酸、生物聚合物和气体。实验结果发现 ,重量百分比为 4 %的糖蜜浓度的营养物最适宜本源微生物生长 ,而且比其它糖蜜浓度下进行试验获得的原油采收率高。由实验的孵化温度可知 :在 2 5℃时 ,本源微生物生长获得很好的采收率。实验结果也发现 ,在储层中存在的碳酸盐 ,由微生物产生的酸中和 ,提供完全发酵的缓冲能力 ,增加CO2 的产量。随着碳酸盐含量的增加 ,原油的采收率也增加。然而 ,碳酸盐的含量进一步增加 ,超过2 0 % ,则对原油采收率不再有影响
At present, the effect of microbial growth on the viscosity, pH and interfacial tension of oil and water is studied by using heavy crude oil (19 ° API gravity) and associated water, and the displacement of crude oil from the water flooding residual oil is studied. Nutrient concentration, incubation temperature and carbonate content. The results show that the use of molasses as a basic nutrient activates the indigenous microbial population in formation water and crude oil to produce biosurfactants, acids, biopolymers and gases. As a result of the experiment, it was found that a nutrient of molasses concentration of 4% by weight was most suitable for growth of a native microorganism and a higher recovery rate of crude oil than that carried out at other molasses concentrations. From the experimental incubation temperature we can see: at 25 ℃, the growth of the source microbial access to good recovery. The experimental results also show that the carbonate present in the reservoir is neutralized by the acids produced by the microorganisms, providing a fully fermentative buffering capacity and increasing CO2 production. As the carbonate content increases, the oil recovery also increases. However, the carbonate content further increases beyond 20%, thus no effect on oil recovery