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目的建立大鼠肾移植急性排斥反应模型,探讨肾移植组织病理机制。方法 78只正常雄性SD大鼠分为正常对照组(A组)15只,模型组(B组)15只,非急性排斥组(C组)16只(以SD大鼠为供体),急性排斥组(D组)16只(以Wistar大鼠为供体),观察术后第7天各组SD大鼠肾功能变化,术后第7天切取移植肾,光镜下观察组织病理改变,参照Banff 97诊断标准,进行急性排斥反应严重程度的半定量评分。结果 C,D组尿素氮、肌酐水平及急性排斥反应半定量评分明显高于A组(P<0.05),D组明显高于C组(P<0.01)。结论 Wistar-SD大鼠移植模型可作为大鼠肾移植急性排斥模型。
OBJECTIVE: To establish a rat model of acute rejection of renal allograft and investigate the pathological mechanism of renal allograft. Methods Totally 78 male SD rats were divided into normal control group (group A) 15, model group (group B) 15, non-acute rejection group (group C) 16 (SD rats as donor), acute Sixteen rats (Wistar rats were used as donors) were excluded from the study. The renal function of SD rats in each group was observed on the 7th day after operation. The graft kidney was excised on the 7th day after operation. The histopathological changes were observed under the light microscope. With reference to the Banff 97 diagnostic criteria, a semi-quantitative score of the severity of acute rejection was obtained. Results The semi-quantitative scores of urea nitrogen, creatinine and acute rejection in groups C and D were significantly higher than those in group A (P <0.05), and those in group D were significantly higher than those in group C (P <0.01). Conclusion Wistar-SD rat transplantation model can be used as acute rejection model in rat kidney transplantation.