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目的建立液相色谱-串联质谱(LC-MS/MS)法测定人全血中阿莫地喹的浓度。方法以稳定同位素化合物氘10-阿莫地喹为内标,全血经直接沉淀后稀释进样分析。以CAPCELL PAK C18 MGIII(100 mm×2.0 mm,5μm)为分析柱,0.2%甲酸的水溶液-0.2%甲酸的乙腈溶液为流动相,梯度洗脱。电喷雾离子源,正离子MRM扫描分析,阿莫地喹和内标离子对分别为m/z 358.2→285.2和m/z 368.2→285.2。结果阿莫地喹在1～100μg.L-1范围内线性关系良好,定量下限为1μg.L-1。批内、批间精密度RSD均小于6%,平均提取回收率在80.9%～87.7%范围内。结论本方法灵敏度高、专一性好、操作简单,可用于人全血中阿莫地喹的检测。
Objective To establish a liquid chromatography-tandem mass spectrometry (LC-MS / MS) method for the determination of amodiaquine in human whole blood. Methods The stable isotope compound deuterium 10-amodipine was used as an internal standard. The whole blood was diluted and analyzed by direct precipitation. The CAPCELL PAK C18 MGIII (100 mm × 2.0 mm, 5 μm) was used as the analytical column. The mobile phase consisted of 0.2% formic acid in water and 0.2% formic acid in acetonitrile, gradient elution. Electrospray ionization, positive ion MRM scanning analysis, amodiaquine and internal standard ion pairs were m / z 358.2 → 285.2 and m / z 368.2 → 285.2, respectively. Results Amodiaquine showed a good linearity in the range of 1 ~ 100μg.L-1 with a lower limit of quantitation of 1μg.L-1. The intra- and inter-batch precision RSDs were less than 6% and the average recovery was within the range of 80.9% to 87.7%. Conclusion The method is sensitive, specific and easy to operate. It can be used for the detection of amodiaquine in human whole blood.