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目的:建立大鼠血浆中木蝴蝶提取物槲皮素含量的分析方法及药代动力学参数。方法:大鼠灌胃给药10 mg/kg后,于不同时间点眼眶取血;采用HPLC法XDB-C18色谱柱,流动相:乙腈-0.1%冰醋酸水溶液进行梯度冼脱,流速:1.00 mL/min,柱温:30℃,检测波长为:278 nm。分析大鼠血浆样品中槲皮素的含量并计算其药动学参数。结果:槲皮素在0.5~22.5μg/mL范围线性关系良好,r=0.9969,提取回收率83.9%~88.5%。日内日间精密度RSD<10.0%。10 mg/kg木蝴蝶提取物槲皮素的tmax为(15.60±4.65)min,Cmax为(0.75±0.12)μg/mL,t1/2为(215.5±102.3)min。结论:该法灵敏度高,操作简便、准确,可用于木蝴蝶提取物大鼠灌胃给药后黄酮类成分的测定及药代动力学研究。
OBJECTIVE: To establish an analytical method and pharmacokinetic parameters of quercetin in the plasma of wood butterfly extract in rats. Methods: Rats were intragastrically administrated with 10 mg / kg of gavage for eye orbital septum at different time points. HPLC was performed on a XDB-C18 column with mobile phase of acetonitrile-0.1% glacial acetic acid and gradient elution at a flow rate of 1.00 mL / min, column temperature: 30 ℃, detection wavelength: 278 nm. The content of quercetin in rat plasma samples was analyzed and their pharmacokinetic parameters were calculated. Results: Quercetin showed good linearity in the range of 0.5-22.5 μg / mL with r = 0.9969. The recoveries of quercetin were 83.9% ~ 88.5%. Intra-day precision RSD <10.0%. The tmax of 10 mg / kg quercetin was (15.60 ± 4.65) min, the Cmax was (0.75 ± 0.12) μg / mL and the t1 / 2 was (215.5 ± 102.3) min. Conclusion: The method has high sensitivity, simple and accurate operation and can be used to determine flavonoids and pharmacokinetics after oral administration of wood butterfly extract.