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目的 :研究沙纳唑对人宫颈癌细胞 (HeLa)的放射增敏作用及其与谷胱甘肽的关系。方法 :用通氮法造成培养细胞乏氧模型 ,给药和60 Coγ照射后用集落形成的方法观察HeLa细胞存活率 ,由单靶多击模型拟合后测出放射增敏比来评价增敏效果 ;用四氧嘧啶紫外分光光度法检测谷胱甘肽含量以探讨增敏作用机制。结果 :SER大于 1.4 ,谷胱甘肽含量随照射剂量和药物浓度的增加而降低 ,尤以乏氧时更为明显。结论 :沙纳唑具有明显的放射增敏作用 ,沙纳唑复合60 Coγ照射使肿瘤谷胱甘肽含量明显降低可能是其放射增敏作用的机制之一
Objective: To study the radiosensitizing effect of zanazole on human cervical cancer cells (HeLa) and its relationship with glutathione. Methods: The model of cultured cells was induced to hypoxia by nitrogen-suppression method. The survival rate of HeLa cells was observed by colony formation after administration and 60 Co gamma irradiation. The radiosensitization ratio was measured after single-target multi-hit model fitting to evaluate sensitization. Effect; Determination of glutathione content by alloxan ultraviolet spectrophotometry to explore the mechanism of sensitization. RESULTS: SER was greater than 1.4, and glutathione content decreased with increasing irradiation dose and drug concentration, especially when hypoxia. Conclusion :Saniazole has obvious radiosensitization effect. The decrease of glutathione content in tumor by tanzanol combined with 60 Coγ irradiation may be one of the mechanisms of radiosensitization.