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目的研究Ras相关区域家族1A(Ras association domain family 1 isoform A,RASSF1A)及结肠腺瘤性息肉病(adenomatosis polyposis coli,APC)基因启动子区CpG甲基化及与前列腺癌(PCa)之间的关系,探索PCa早期诊断的检测方法。方法收集60例PCa和40例前列腺增生(BPH)病例的组织标本及其相关的临床指标。应用亚硫酸氢盐修饰后测序法检测PCa组织及BPH组织中RASSF1A、APC基因启动子区CpG甲基化情况。结果 PCa组RASSF1A、APC基因CG位点甲基化率高于BPH组(60.8%vs 14%,48.84%vs 1.19%,P<0.05);基因甲基化率与PSA、Gleason评分、病理分期和PCa危险分期关系密切(P<0.01);RASSF1A及APC基因甲基化联合检测用于判断PCa及BPH的敏感度和特异度是95.74%和82.9%。结论 RASSF1A、APC基因启动子区甲基化与PCa发生及发展有关,其甲基化率的变化与PCa的危险分期关系密切。检测前列腺组织中相关基因甲基化状态,有望成为诊断早期PCa的一种方法。
Objective To investigate the relationship between CpG methylation in promoter region of Ras association domain family 1 isoform A (APA) and adenocarcinoma polyposis coli (APC) and its relationship with prostate cancer (PCa) Relationship, to explore the detection of PCa early detection methods. Methods Tissue samples of 60 cases of PCa and 40 cases of benign prostatic hyperplasia (BPH) were collected and their related clinical indexes were collected. The methylation of CpG methylation in promoter region of RASSF1A and APC genes in PCa and BPH tissues was detected by bisulfite modified sequencing. Results The methylation rates of RASSF1A and APC genes in PCa group were higher than those in BPH group (60.8% vs 14%, 48.84% vs 1.19%, P <0.05). The methylation rates of the genes were positively correlated with PSA, Gleason score, pathological stage (P <0.01). The combined methylation of RASSF1A and APC genes was used to determine the sensitivity and specificity of PCa and BPH were 95.74% and 82.9%, respectively. Conclusion The promoter methylation of RASSF1A and APC genes is related to the occurrence and development of PCa. The methylation rate of RASSF1A and APC is closely related to the risk staging of PCa. Detecting the methylation status of related genes in prostate tissue is expected to become a method to diagnose early PCa.